Evaluation of the Etest for susceptibility testing of Mycoplasma hominis and Ureaplasma urealyticum

The Etest was used for antibiotic susceptibility testing of Mycoplasma homi nis and Ureaplasma urealyticum isolates and the results were compared with those obtained with the broth microdilution method. For 50 clinical isolate s of M, hominis the MICs of doxycycline, ofloxacin and ciprofloxacin agreed within +/- one dilution and +/- two dilutions in 82-98% and 98-100% of cas es, respectively. The MICs of erythromycin, azithromycin, doxycycline, oflo xacin and ciprofloxacin were evaluated for 50 clinical isolates of U, ureal yticum, The corresponding levels of agreement were 70-98% and 94-100%, resp ectively. Reference isolates M. hominis PG-21 and U. urealyticum T-960 were also used. The Etest seems to be an alternative method for determination o f MICs of antibiotics with M. hominis and U. urealyticum.