Regulation of muscarinic acetylcholine receptor sequestration and functionby beta-arrestin

After activation, agonist-occupied G; protein-coupled receptors are phospho rylated by G protein-coupled receptor kinases and bind cytosolic beta-arres tins, which uncouple the receptors from their cognate G;proteins, Recent st udies on the beta(2)-adrenerse receptor have demonstrated that beta-arresti n also targets the receptors to clathrin-coated pits for subsequent interna lization and activation of mitogen-activated protein kinases, We and others have previously shown that muscarinic acetylcholine receptors (mAChRs) of the mi, m3, and m4 subtype require functional dynamin to sequester into HEK -293 tsA201 cells, whereas m2 mAChRs sequester in a dynamin-independent man ner. To investigate the role of beta-arrestin in mAChR sequestration, we de termined the effect of overexpressing beta-arrestin-1 and the dominant-nega tive inhibitor of beta-arrestin-mediated receptor sequestration, beta-arres tin-1 V53D, on mAChR sequestration and function. Sequestration of mi, m3, a nd m4 mAChRs was suppressed by 60-75% in cells overexpressing beta-arrestin -1 V53D, whereas m2 mAChR sequestration was affected by less than 10%. In a ddition, overexpression of beta-arrestin-1 V53D as well as dynamin K44A sig nificantly suppressed mi mAChR-mediated activation of mitogen-activated pro tein kinases, Finally, we investigated whether mAChRs sequester into clathr in-coated vesicles by overexpressing Hub, a dominant-negative clathrin muta nt. Although sequestration of mi, m3, and m4 mAChRs was inhibited by 50-70% , m2 mAChR sequestration was suppressed by less than 10%, We conclude that mi, m3, and m4 mAChRs expressed in HEK-293 tsA201 cells sequester into clat hrin-coated vesicles in a beta-arrestin- and dynamin-dependent manner, wher eas sequestration of m2 mAChRs in these cells is largely independent of the se proteins.