Md. Sanchezsuarez et A. Costagarcia, COMPARATIVE VOLTAMMETRIC STUDIES OF IMMUNOGLOBULINS AND DNP-LABELED IMMUNOGLOBULINS, Talanta, 44(5), 1997, pp. 909-915
A comparative electrochemical study of human immunoglobulins IgG(1) an
d IgG(3) carried out at a hanging mercury drop electrode shows that me
chanisms other than the reduction of interchain disulphide linkages ar
e responsible for the cathodic peaks observed for such proteins. Consi
dering that the nature of the electrochemical process observed for imm
unoglobulins are poorly defined and not fully understood, a new approa
ch to the electrochemical determination of such proteins, involving th
e use of 2,4-dinitrophenol (DNP) as a label, has been developed. Dynam
ic linear ranges of nearly two magnitudes and detection limits below 1
0(-10) M were achieved. (C) 1997 Elsevier Science B.V.