High-performance liquid chromatographic assay of (+/-)-lactic acid and itsenantiomers in calf serum

Two high-performance liquid chromatographic methods are described for the d etermination of lactic acid and its enantiomers in calf serum. A 300 X 8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50 X 4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area r atios and serum concentrations over a range of 0.5 to 20 mM, based on 100 m u l bovine serum. Recovery was complete. Inter and within-batch bias and re lative standard deviation were <15%. (C) 1999 Elsevier Science B.V. All rig hts reserved.