Semi-automated solid-phase extraction procedure for the high-performance liquid chromatographic determination of alinastine in biological fluids

A solid-phase extraction (SPE) method for sample clean-up followed by a rev ersed-phase HPLC procedure for the assay of alinastina (pINN) in biological fluids is reported. The effects of the sample pH, composition of the washi ng and elution solvents and the nature of the SPE cartridge on recovery wer e evaluated. The selectivity of SPE was examined using spiked rat urine and plasma samples and the CH and PH cartridges gave rise to the cleanest extr acts. The recoveries obtained in spiked rat urine and plasma samples were 9 1.2+/-2.7 and 99.9+/-2.8%, respectively. The proposed SPE method coupled of f-line with a reserved-phase HPLC system with fluorimetric detection was ap plied to the quantitation of alinastine in real rat urine samples. The anal ytical method was also applied and validated for the determination of alina stine in dog plasma. The recovery from spiked dog plasma samples using the PH cartridge was around 65%. The within-day and between-day precisions were 7 and 12%, respectively. The detection and quantitation limits in dog plas ma were 0.024 and 0.078 mu g/ml, respectively. (C) 1999 Elsevier Science B. V. All rights reserved.