Restriction analysis of the ITS region for characterization of the Debaryomyces species

Authors
Ramos, JP Valente, P Hagler, AN Leoncini, O
Citation
Jp. Ramos et al., Restriction analysis of the ITS region for characterization of the Debaryomyces species, J GEN A MIC, 44(6), 1998, pp. 399-404
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
ISSN journal
00221260 → ACNP
Volume
44
Issue
6
Year of publication
1998
Pages
399 - 404
Database
ISI
SICI code
0022-1260(199812)44:6<399:RAOTIR>2.0.ZU;2-8
Abstract
The internal transcribed spacer (ITS) region of rDNA was used for taxonomic inferences in ascomycetous yeasts. The Debaryomyces species had a 640-690 ITS size. The analyzed Candida species can be differentiated by its distinc t ITS size. The enzymatic digestion of the ITS region show large homogeneit y in Debaryomyces, with polymorphism for only two enzymes. The ITS size and the enzymatic restriction method were used in Brazilian isolates, detectin g some Debaryomyces misidentifications in cultures previously identified by conventional methods.