Comprehensive evaluation of isoprenoid biosynthesis regulation in Saccharomyces cerevisiae utilizing the Genome Reporter Matrix (TM)

Gene expression profiling is rapidly becoming a mainstay of functional geno mic studies. However, there have been relatively few studies of how the dat a from expression profiles integrate with more classic approaches to examin e gene expression. This study used gene expression profiling: of a portion of the genome of Saccharomyces cerevisiae to explore the impact of blocks i n the isoprenoid biosynthetic pathway on the expression of genes and the re gulation of this pathway. Approximately 50% of the genes whose expression w as altered by blocks in isoprenoid biosynthesis were genes previously known to participate in the pathway. In contrast to this simple correspondence, the regulatory patterns revealed by different blocks, and in particular by antifungal azoles, was complex in a manner not anticipated by earlier studi es.