Np. Dantuma et al., An insect homolog of the vertebrate very low density lipoprotein receptor mediates endocytosis of lipophorins, J LIPID RES, 40(5), 1999, pp. 973-978
A novel member of the low density lipoprotein (LDL) receptor family was ide
ntified, which is expressed in locust oocytes, fat body, brain, and midgut,
This receptor appeared to be a homolog of the mammalian very lo iv density
lipoprotein receptor as it contains eight cysteine-rich repeats in its put
ative ligand-binding domain. When transiently expressed in COS-7 or stably
expressed in LDL receptor-deficient CHO tells, the receptor mediates endocy
tic uptake of high density lipophorin (HDLp), an abundant lipoprotein in th
e circulatory compartment of insects. Moreover, in the latter cell line, we
demonstrated that an excess of unlabeled HDLp competed with fluorescent la
beled HDLp for uptake whereas an excess of human LDL did not affect uptake.
Expression of the receptor mRNA in fat body cells is down-regulated during
adult development, which is consistent with the previously reported down-r
egulation of receptor-mediated endocytosis of lipophorins in fat body tissu
e (Dantuma, N. P., M.A.P. Pijnenburg, J. H. B. Diederen, and D.J. Van der H
orst. 1997,J, Lipid Res. 38: 254-265). The expression of this receptor in v
arious tissues that internalize circulating lipophorins and its capability
to mediate endocytosis of HDLp indicate that this novel member of the LDL r
eceptor family may function as an endocytic lipophorin receptor in vivo.