A STUDY OF THE BEHAVIOR OF SOME NEW COLUMN MATERIALS IN THE CHROMATOGRAPHIC ANALYSIS OF CATHARANTHUS ALKALOIDS

Authors
THEODORIDIS G PAPADOYANNIS I HERMANSLOKKERBOL A VERPOORTE R
Citation
G. Theodoridis et al., A STUDY OF THE BEHAVIOR OF SOME NEW COLUMN MATERIALS IN THE CHROMATOGRAPHIC ANALYSIS OF CATHARANTHUS ALKALOIDS, Chromatographia, 45, 1997, pp. 52-58
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
45
Year of publication
1997
Supplement
S
Pages
52 - 58
Database
ISI
SICI code
0009-5893(1997)45:<52:ASOTBO>2.0.ZU;2-A
Abstract
New column-packing materials specially designed for the HPLC analysis of basic compounds have been tested for the analysis of Catharanthus a lkaloids. Mobile phase optimization was performed for each column test ed. The influence of mobile phase pH, nature and content of the organi c modifier and salt concentration on retention, selectivity and resolu tion was studied. An important factor in the separation proved to be t he pH of the eluent, because of the widely different pK(a), values of the analytes. Complete separation was easily achieved on ODS columns, but polymeric materials also gave acceptable results.