Js. Pedersen et Jj. Boomsma, Multiple paternity in social Hymenoptera: estimating the effective mate number in single-double mating populations, MOL ECOL, 8(4), 1999, pp. 577-587
Several hypotheses have been advanced to explain the widespread occurrence
of multiple mating by queens in eusocial bees, wasps and ants. In order to
test competing hypotheses, it is essential to obtain an accurate descriptio
n of the distribution of mate number and the mates' respective contribution
s to the brood in populations under study. In this study we examine current
methods for estimating the effective mate number and other paternity frequ
ency parameters in populations dominated by single and double mating of que
ens, based on genetical data from parent-offspring combinations. Estimation
errors are due to limited sample size of offspring (nonsampling error) and
limited variation of genetic markers (nondetection error). We demonstrate
that limited sample size of offspring leads to an underestimation of the pa
ternity contribution from the majority male of double-mated queens (the pat
ernity skew or sperm bias), thus overestimating the effectiveness of double
matings and underestimating the population frequency of double-mated queen
s. We propose a calculation procedure which avoids this bias. Furthermore,
we propose a general method to correct simultaneously for errors from nonde
tection and nonsampling when estimating the population frequency of double-
mated queens. The calculation procedures presented are designed for male-ha
plodiploid social Hymenoptera but some can be applied directly to studies o
f diplo-diploid mating systems where single and double mating prevail. This
has particular relevance to vertebrate studies where the number of offspri
ng studied per mother is invariably low. The remaining calculation procedur
es are conceptually widely applicable, but details would have to be modifie
d for other than haplodiploid mating systems.