Production of goats by somatic cell nuclear transfer

In this study, we demonstrate the production of transgenic goats by nuclear transfer of fetal somatic cells. Donor karyoplasts were obtained from a pr imary fetal somatic cell line derived from a 40-day transgenic female fetus produced by artificial insemination of a nontransgenic adult female with s emen from a transgenic male. Live offspring were produced with two nuclear transfer procedures. In one protocol, oocytes at the arrested metaphase II stage were enucleated, electrofused with donor somatic cells, and simultane ously activated. In the second protocol, activated in vivo oocytes were enu cleated at the telophase II stage, electrofused with donor somatic cells, a nd simultaneously activated a second time to induce genome reactivation. Th ree healthy identical female offspring were born. Genotypic analyses confir med that all cloned offspring were derived from the donor cell line. Analys is of the milk of one of the transgenic cloned animals showed high-level pr oduction of human antithrombin III, similar to the parental transgenic line .