Synthesis of a monocharged peptide nucleic acid (PNA) analog and its recognition as substrate by DNA polymerases

Authors
Lutz, MJ Will, DW Breipohl, G Benner, SA Uhlmann, E
Citation
Mj. Lutz et al., Synthesis of a monocharged peptide nucleic acid (PNA) analog and its recognition as substrate by DNA polymerases, NUCLEOS NUC, 18(3), 1999, pp. 393-401
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
3
Year of publication
1999
Pages
393 - 401
Database
ISI
SICI code
0732-8311(1999)18:3<393:SOAMPN>2.0.ZU;2-U
Abstract
The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coup led to the secondary nitrogen of 2-(2-amino-ethylamino)ethanol i s described. This monomer can be used to attach a deoxynucleotide to the ca rboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PN A primer is recognized as a substrate by various DNA polymerases.