Recognition of 2 '-deoxyisoinosine triphosphate by the Klenow fragment of DNA polymerase I

Authors
Beaussire, JJ Pochet, S
Citation
Jj. Beaussire et S. Pochet, Recognition of 2 '-deoxyisoinosine triphosphate by the Klenow fragment of DNA polymerase I, NUCLEOS NUC, 18(3), 1999, pp. 403-410
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
3
Year of publication
1999
Pages
403 - 410
Database
ISI
SICI code
0732-8311(1999)18:3<403:RO2'TB>2.0.ZU;2-F
Abstract
The capability of the Klenow fragment deficient in 3'-->5' exonuclease acti vity (KFexo(-)) to incorporate 2'-deoxyisoinosine (disoI) opposite canonica l bases and 5-methyl-2-deoxyisocytidine (d(5Me)isoC) was examined. The trip hosphate of disoI is incorporated opposite d(5Me)isoC designed as its pairi ng partner. It is also incorporated opposite T and G according to a pairing scheme involving its minor tautomeric form (the (OH)-H-2 tautomer).