Oligonucleotides quality control analysis in free solution by capillary electrophoresis at acidic pH.

Authors
Mangano, MF Battaglia, C Salani, G Bernardi, LR De Bellis, G
Citation
Mf. Mangano et al., Oligonucleotides quality control analysis in free solution by capillary electrophoresis at acidic pH., NUCLEOS NUC, 18(3), 1999, pp. 469-474
Citations number
11
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
3
Year of publication
1999
Pages
469 - 474
Database
ISI
SICI code
0732-8311(1999)18:3<469:OQCAIF>2.0.ZU;2-L
Abstract
A new method for fast, automated and inexpensive oligonucleotides analysis by capillary electrophoresis at low pH is presented. This method does not n eed any sieving media to resolve a mixture of polynucleotides which are ana lysed in free solution and separated on the base of composition and not len gth. This technique has been used to test a large set of standard and modif ied oligonucleotides thus to be applied in oligos routine quality control.