Specificity and sensitivity of random amplified polymorphic DNA analysis for the identification of single larvae of Trichinella after experimental infection of pigs
E. Pozio et al., Specificity and sensitivity of random amplified polymorphic DNA analysis for the identification of single larvae of Trichinella after experimental infection of pigs, PARASIT RES, 85(6), 1999, pp. 504-506
Single muscle larvae (ML) of Trichinella belonging to 5 species and 2 genot
ypes collected from 126 experimentally infected pigs and preserved in 75% e
thyl alcohol at room temperature were identified by random amplified polymo
rphic DNA (RAPD) analysis to evaluate the specificity and sensitivity of th
is assay. A double-blind protocol was used to ensure that the interpretatio
n of electrophoretic patterns after RAPD amplification was not influenced b
y an expected result. RAPD analysis of one larva from each sample showed a
sensitivity of 100% when only samples with undamaged DNA were considered, a
nd the specificity resulted in an 88% match with the species or genotype th
at had been used to infect pigs, whereas the identification reached 100% sp
ecificity when an additional one to four ML were examined.