Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

Authors
Haruta, M Murata, M Kadokura, H Homma, S
Citation
M. Haruta et al., Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees, PHYTOCHEM, 50(6), 1999, pp. 1021-1025
Citations number
28
Categorie Soggetti
Agricultural Chemistry","Animal & Plant Sciences
Journal title
PHYTOCHEMISTRY
ISSN journal
00319422 → ACNP
Volume
50
Issue
6
Year of publication
1999
Pages
1021 - 1025
Database
ISI
SICI code
0031-9422(199903)50:6<1021:IAMCOP>2.0.ZU;2-V
Abstract
An antibody raised against apple polyphenol oxidase (PPO) cross-reacted wit h PPOs from Japanese pear (Pyrus pyrifolia). pear (Pyrus communis), peach ( Prunus persica); Chinese quince (Pseudocydonia sinensis) and Japanese loqua t (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO g enes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PP Os showed higher activities when assayed :ir pH4 than at pH6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically s imilar. (C) 1999 Elsevier Science Ltd. All rights reserved.