Enhanced expression and differential inducibility of soybean chalcone synthase genes by supplemental UV-B in dark-grown seedlings

By developing gene-specific RT-PCR and using filters to allow transmission down to 290 nm (UV-B+) or blocking all radiation below 320 nm (UV-B-), the effect of UV-B+ and UV-B- light on expression of each of the presently know n seven members of soybean chalcone synthase (CHS) gene family in dark-grow n seedlings was analyzed. Dark expression was detectable already in 18 h da rk-germinating embryos, with progressive increases on successive days, sugg esting that chs belongs to a class of genes expressed very early during ger mination, and that the expression at this stage is either constitutive or i nduced by non light-dependent factors present in the seed or made available following imbibition. Exposure of 18 h dark-germinating embryos to UV-B- o r to UV-B+ light did not lead to an increase in chs signal. However, the 24 h dark-germinating embryos showed a distinct effect of UV-B+ interestingly coinciding with the stage when the head of seedlings was in the process of being pushed up above ground by stem elongation, suggesting the possibilit y of a developmental switch modulating the appearance of UVB response. The response to UV-B- was most prominent in chs1 and almost silent in chs2, whi le the up-regulation by UV-B+ was most prominent in chs5 and chs6 and much less so in chs2. Interestingly, chs2 was noted to be the only member of the Gmchs gene family devoid of H box, raising the possibility that the H-box may be a good indicator of the photo-inducibility of a chs gene.