LACCASE ISOENZYME PATTERNS OF EUROPEAN ARMIALLARIA SPECIES FROM CULTURE FILTRATES AND INFECTED WOODY PLANT-TISSUES

Polyacrylamide isoelectric focusing with specific staining for laccase activity was used to characterize laccase from European Armillaria sp ecies (Armillaria ostoyae, Armillaria mellea, Armillaria gallica, Armi llaria cepistipes). The enzyme was extracted from culture media either supplemented, or not, with pine sawdust, and also from Pinus pinaster naturally infected by A. ostoyae, or artificially inoculated with A. mellea and A. ostoyae Some differences in banding patterns were found for Armillaria isolates according to the species and the culture media , but a common band at pI=3.4 was found in ail the extracts tested, in dependently of their origin (culture filtrate or wood).