Immunolocation of antisperm monoclonal antibody 6B10 and corresponding antigen

An antisperm monoclonal antibody 6B10 was produced by hybridoma technique o f the isotype IgG. The monoclonal antibody was purified by means of ammoniu m sulfate precipitation and protein A-Sepharose C1-4B affinity chromatograp hy. SDS polyacrylamide gel electrophoresis was used to evaluate the purity of the antibody. Evaluation of the sperm acrosomal status was determined by chlortetracycline (CTC) staining. It was found that monoclonal antibody 6B 10 can inhibit the sperm acrosome reaction induced by progesterone. The cor responding antigen recognized by monoclonal antibody 6B10 was located on th e plasma membrane of the sperm acrosome by indirect immunofluorescent micro scopy and immunoelectronmicroscopy. Sperm protein was extracted by 1%. Trit on X-100. The molecular weight of the antigen is 50 ku, detected by Western blot. The antigen is a key protein in the sperm acrosome reaction and may be the receptor of progesterone on the sperm acrosome. It may either be dev eloped as a candidate contraceptive vaccine or be used as a tool in pest/ro dent management.