Improvement in the intestinal processes of hydroelectrolytic absorption and secretion in abdominal pathologies of surgical interest treated with SMS 201-995: Experimental protocol

The hypothesis that octreotide can improve the intestinal absorption and se cretion processes in a mixed group of intestinal pathologies, and that this effect varies according to the pathology in question, was tested, One hund red and twenty Wistar rats were randomly assigned to six pathology groups c onsisting of three intestinal occlusions including (1) complete, (2) partia l, and (3) complete with strangulation, and three mesenteric vascular occlu sions including (4) partial permanent, (5) total permanent, and (6) total t emporary. Each group contained ten control and ten treated rats. The treate d animals received octreotide (100 mu g/kg body weight) while the controls were given the same quantity of saline solution every 8 h, After the observ ation period, the contents of the small intestine were extracted and its vo lume measured before and after centrifugation; the concentration and total content of Na, K, Cl, and bicarbonate was then analyzed. Samples of all the intestines at specific distances from the lesion zone were treated and sta ined, and then evaluated according to a specific score to quantify the lesi ons. The concentration and contents of electrolytes in the intestine and it s volume (before and after centrifugation) were lower in the treated animal s, but varied according to the pathology, There was a nonadditive influence between the type of pathology and treatment for the four electrolytes and intestinal volume. The effects of the drug make it directly or indirectly p ossible to decrease the intestinal lesions to improve the absorption-secret ion processes. Octreotide acts on intestinal secretion and absorption in al l the pathologies analyzed except for total permanent intestinal ischemia. Its action also varies according to the type of pathology involved.