Localization of an nonpalpable colonic lesion at the time of colectomy usua
lly requires intraoperative colonoscopy. The use of ultrasound to locate th
e lesion has not been described. A soft bowel clamp is placed above the exp
ected location of the lesion and a catheter placed in the anus. Saline is t
hen instilled into the colon and rectum. The lesion is located by ultrasoun
d scan of the fluid filled colon with the probe placed on the serosal surfa
ce. Refinement of the technique was performed on resected colonic specimens
. An in vivo trial was then performed with rapid and accurate localization
of the lesion for resection. Intraoperative ultrasound of the colon can acc
urately localize nonpalpable colonic lesions and is an alternative to curre
ntly available techniques of localization.