P. Hernandez et al., Development and characterization of Hordeum chilense chromosome-specific STS markers suitable for wheat introgression and marker-assisted selection, THEOR A GEN, 98(5), 1999, pp. 721-727
RAPD markers were developed for octoploid x Tritordeum (amphiploid Hordeum
chilense x Triticum aestivum) and its parents. Addition lines were used to
identify specific RAPD markers for the Hordeum chilense chromosomes detecta
ble in a wheat background. Twelve RAPD fragments have been cloned, sequence
d and converted into STS markers. Eleven of these STSs have maintained both
the chromosome specificity and the possibility of detection in a wheat bac
kground. The use of these markers in multiplexed PCRs facilitates both the
efficient and reliable screening of new addition lines as well as the monit
oring of introgression of H. chilense in bread and durum wheat.