Characterisation of a cytoplasmic male-sterile hybrid line between Lycopersicon peruvianum Mill. x Lycopersicon pennellii Corr. and its crosses with cultivated tomato

The cytoplasmic male-sterile (CMS) line CMS-pennellii (BC10P2 L. peruvianum x L. pennellii) and its complex hybrids with L, esculentum were studied. T he established sterility was classified as the sporogenous type. As a resul t of the interaction of the genome of L. pennellii and the cytoplasm of L. peruvianum clear changes were established in the profiles of malic enzyme a nd esterase. Restriction fragment length polymorphism (RFLP) was detected b etween the mitochondrial (mt) genomes of CMS-pennellii and the cytoplasm do nor, L, peruvianum, for two mtDNA probes: atpA and nad3. The established di fferences in the isozyme pattern and mt genomes are considered as useful ma rkers to distinguish fertile and sterile plants. A breakthrough in the unil ateral incompatibility of CMS-pennellii and the incorporation of the genome of L. esculentum on a CMS background is reported. The analysis of the comp lex hybrids assumes the interaction of two dominant genes - a maintainer ge ne from L. pennellii and a restorer gene from cultivated tomato. The hybrid s produced with L. esculentum provide the basis for the development of a CM S system in cultivated tomato.