Detection of single-stranded cohesive ends in the genome of Bacillus thuringiensis temperate phage KK-88

Cohesive ends (cos sites) were detected in the genome of temperate KK-88 ph age in Bacillus thuringiensis after analysis of the phage DNA generated by the 3'-5' exonuclease activity of Klenow fragment of DNA polymerase I. In a ddition, unlike the 5'-protruding ends of coliphage lambda genome, the ends of KK-88 phage genome were found to be 3'-protruding. The restriction map of the phage genome was also constructed on the basis of position of the co s fragments.