Protein adsorption dissociation constants in various types of biochromatography

The equilibrium kinetics, adsorption and desorption of various proteins on chromatographic adsorbents have been widely studied and published in the li terature. What is lacking is a comprehensive review of the available inform ation. In this article the equilibrium data has been summarized and present ed in the form of dissociation constants. This summary leads to a chart of relative protein adsorption strength of various types of chromatography in terms of dissociation constants. Some of the factors affecting dissociation constants such as ligand concentration, pH, ionic strength and temperature have also been discussed. The results of this study can be used as importa nt information for design, development, scale up and optimization of chroma tographic processes for protein separations.