We sampled the hyporheic cyclopoid copepods of 14 unpolluted sites in the e
astern United States to test whether community composition differed between
glaciated and unglaciated sites. We found 31 species of cyclopoids in all,
over half of which were new to science or only recently described. All of
these newly discovered species were interstitial specialists ("stygobionts"
) living in the unglaciated Southeast, suggesting that many new species of
hyporheic cyclopoids remain to be found in this region. Glaciated sites con
tained fewer species of interstitial specialists (but not fewer species of
generalists) and fewer narrowly endemic species than unglaciated sites. Fur
ther, ordination results suggest that there is a break in species compositi
on near the glacial border. In contrast, local ecological conditions had mu
ch weaker effects on species composition. We also found strong differences
between interstitial specialists and generalists in local distributions wit
hin our study sites and in range size-abundance relationships. Our results
thus show that glaciation has strong residual effects on the composition of
present-day communities of hyporheic cyclopoids.