Modeling and simulation of protein adsorption in permeable chromatographicpackings: a double linear driving force model

Permeable large-pore packings have many applications, particularly in perfu sion chromatography for bioseparations. The major objectives of this paper are: (i) to develop a double linear driving force (LDF) model based on line ar driving force approximations inside throughpores and microparticles to s imulate dynamic nonlinear adsorption in columns having spherical permeable chromatographic packings with a bidisperse pore structure; and (ii) to anal yze the performance of this simple model to simulate breakthrough and eluti on curves for bovine serum albumin adsorption on POROS Q/M large-pore parti cles (PerSeptive Biosystems, Cambridge, MA, USA), in comparison with a more realistic and difficult model (intraparticle diffusion/convection model) w hich is also presented in this paper. Model results obtained for several va lues of the intraparticle Peclet number, lambda, resulting from the convect ive flow in the throughpores show the very reasonable performance of the do uble LDF model in simulating breakthrough and elution curves of proteins, p articularly for higher values of lambda. This finding is of relevance due t o the remarkable saving in computation time afforded with the double LDF mo del. (C) 1999 Elsevier Science S.A. All rights reserved.