Of all NMR observable isotopes F-19 is the one perhaps most convenient for
studies on biodegradation of environmental pollutants. The reasons underlyi
ng this potential of F-19 NMR are discussed and illustrated on the basis of
a study on the biodegradation of fluorophenols by four Rhodococcus strains
. The results indicate marked differences between the biodegradation pathwa
ys of fluorophenols among the various Rhodococcus species. This holds not o
nly for the level and nature of the fluorinated biodegradation pathway inte
rmediates that accumulate, but also for the regioselectivity of the initial
hydroxylation step. Several of the Rhodococcus species contain a phenol hy
droxylase that catalyses the oxidative defluorination of ortho-fluorinated
di- and trifluorophenols. Furthermore, it is illustrated how the F-19 NMR t
echnique can be used as a tool in the process of identification of an accum
ulated unknown metabolite, in this case most likely 5-fluoromaleylacetate.
Altogether, the F-19 NMR technique proved valid to obtain detailed informat
ion on the microbial biodegradation pathways of fluorinated organics, but a
lso to provide information on the specificity of enzymes generally consider
ed unstable and, for this reason, not much studied so far.