F-19 NMR study on the biodegradation of fluorophenols by various Rhodococcus species

Citation
Vs. Bondar et al., F-19 NMR study on the biodegradation of fluorophenols by various Rhodococcus species, BIODEGRADAT, 9(6), 1998, pp. 475-486
Citations number
37
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIODEGRADATION
ISSN journal
09239820 → ACNP
Volume
9
Issue
6
Year of publication
1998
Pages
475 - 486
Database
ISI
SICI code
0923-9820(1998)9:6<475:FNSOTB>2.0.ZU;2-9
Abstract
Of all NMR observable isotopes F-19 is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlyi ng this potential of F-19 NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains . The results indicate marked differences between the biodegradation pathwa ys of fluorophenols among the various Rhodococcus species. This holds not o nly for the level and nature of the fluorinated biodegradation pathway inte rmediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hy droxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the F-19 NMR t echnique can be used as a tool in the process of identification of an accum ulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the F-19 NMR technique proved valid to obtain detailed informat ion on the microbial biodegradation pathways of fluorinated organics, but a lso to provide information on the specificity of enzymes generally consider ed unstable and, for this reason, not much studied so far.