Construction and characterization of Escherichia coli disruptants defective in the yaeM gene

Authors
Kuzuyama, T Takahashi, S Seto, H
Citation
T. Kuzuyama et al., Construction and characterization of Escherichia coli disruptants defective in the yaeM gene, BIOS BIOT B, 63(4), 1999, pp. 776-778
Citations number
22
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
63
Issue
4
Year of publication
1999
Pages
776 - 778
Database
ISI
SICI code
0916-8451(199904)63:4<776:CACOEC>2.0.ZU;2-Y
Abstract
Escherichia coli disruptants defective in the yaeM gene, which is located a t 4.2 min on the chromosome map, were constructed and characterized. The di sruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C -methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the no nmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.