Natural zeolite was added to the sequencing batch reactors to assess its ro
le in ammonium exchange. Even though the biofilm was fully developed on the
zeolite surface, ammonium removal and the biological regeneration of the z
eolite occurred constantly during the anoxic-fill phase and the reaction ph
ase, respectively. However, the specific nitrification rate of the bio-floc
culated zeolite was lower than that observed in the control on account of t
he limited ammonium release to the liquid phase.