Kk. Srivastava et al., A recombinant cellulolytic Escherichia coli: Cloning of the cellulase geneand characterization of a bifunctional cellulase, BIOTECH LET, 21(4), 1999, pp. 293-297
A genomic library of Bacillus subtilis CD4 was constructed in Escherichia c
oli JM83. A clone designated as E. coli pBcelR was identified which formed
blue colony in presence of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranosid
e (X-Glu) and hydrolysed carboxymethyl cellulose (CMC). The clone E. coli (
pBcelR) expressed both cellobiase and endoglucanase activities and containe
d an insert of 1.2 kb. E. coli pBcelR encoded a protein of 12.9 kDa which w
as endowed with bifunctional (endoglucanase and cellobiase) activities. In
recombinant E. coli, the encoded protein and enzyme activity were localized
in periplasm. Recombinant E. coli pBcelR utilized CMC, cellobiose and solu
ble cellulose as sole carbon source.