Production of Oenococcus oeni biomass to induce malolactic fermentation inwine by control of pH and substrate addition

To increase the commercial production of Oenococcus oeni strains to be used for biological deacidification of wines, substrates addition and pH contro l have been optimized. The highest biomass yield of Oenococcus oeni (Y = 6. 9 mg mmol(-1) sugar) was obtained when 55 mmol glucose l(-1) and 30 mmol fr uctose l(-1) were added both to the culture medium, and the pH was controll ed at 4.8. Fructose was used as carbon and energy source, but also as elect ron acceptor improving the ability to reoxidize NAD(P)H.