A genetic physical map in river buffalo (Bubalus bubalis, 2n=50)

This study reports the first entire genetic physical map in river buffalo c hromosomes by ISM-techniques. Thirty-six loci (mostly mapped by FISH), of w hich 19 were expressed genes and 17 were DNA-segments (cosmid derived-micro satellites), were localized in specific chromosomes. At least one molecular marker from all 31 bovine syntenic groups was assigned to each river buffa lo chromosome. This allowed tentative assignment of all bovine syntetic gro ups to river buffalo chromosomes, also in consideration of high degree of c hromosome banding homologies between the two species and that all mapped lo ci in river buffalo chromosomes were at the same cattle homoeologous chromo somes and chromosome bands. By adding markers from bovine U1, U3, U7, U10, U13, U16, U17, U25, U28 and U29 assigned to the river buffalo genome by the somatic cell hybrid techniq ue, a total of 54 markers is assigned to river buffalo genome.