Application of charge transfer reaction in the pharmaceutical analysis - Determination of chlorhexidine acetate

A spectrophotometric method for the determination of chlorhexidine acetate is described. The reaction between chlorhexidine acetate and chloranil took place in an alcohol-acetone solution at room temperature. The composition of the charge transfer complex is 1:2. Beer's law is obeyed in the concentr ation range of 15270 mu g.mL(-1) with correlation coefficient 0.9995. The a pparent molar absorptivity is 2.21 x 10(3) L.mol(-1)'.cm(-1) at 412 nm. The method is accurate (with a recovery of 100 +/- 1.6%) and precise (RSD = 1. 0%). It was successfully applied to determine chlorhexidine acetate in supp ository or disinfectant solution.