Analysis of deprenyl metabolites in the rat brain using HPLC-ESMS

Methylamphetamine and amphetamine, the two major metabolites of deprenyl in the rat brain were analyzed using HPLC method combined with electrospray-m ass spectrometer. (-)-Deprenyl and (+)-deprenyl were orally administered to rats either in a single dose of 10 mg/kg, or three times a week for three weeks. The metabolites were determined in four different parts of the rat b rain, such as in the frontal cortex, corpus striatum, hippocampus, and hypo physis. The ratio of methylamphetamine to amphetamine was also compared aft er (-)-deprenyl and (+)-deprenyl treatments.