Insulin action on muscle protein kinetics and amino acid transport during recovery after resistance exercise

We have determined the individual and combined effects of insulin and prior exercise on leg muscle protein synthesis and degradation, amino acid trans port, glucose uptake, and alanine metabolism. Normal volunteers were studie d in the postabsorptive state at rest and about 3 h after a heavy leg resis tance exercise routine. The leg arteriovenous balance technique was used in combination with stable isotopic tracers of amino acids and biopsies of th e vastus lateralis muscle. Insulin was infused into a femoral artery to inc rease the leg insulin concentrations to high physiologic levels without sub stantively affecting the whole-body level. Protein synthesis and degradatio n were determined as rates of intramuscular phenylalanine utilization and a ppearance, and muscle fractional synthetic rate (FSR) was also determined. Leg blood flow was greater after exercise than at rest (P < 0.05). Insulin accelerated blood now at rest but not after exercise (P < 0.05). The rates of protein synthesis and degradation were greater during the postexercise r ecovery (65 +/- 10 and 74 +/- 10 nmol min(-1) . 100 ml(-1) leg volume, resp ectively) than at rest (30 +/- 7 and 46 +/- 8 nmol . min(-1) . 100 ml(-1) l eg volume, respectively; P < 0.05). Insulin infusion increased protein synt hesis at rest (51 +/- 4 nmol . min(-1) . 100 ml(-1) leg volume) but not dur ing the postexercise recovery (64 +/- 9 nmol . min(-1) . 100 ml(-1) leg vol ume; P < 0.05). Insulin infusion at rest did not change the rate of protein degradation (48 +/- 3 nmol . min(-1) . 100 ml(-1) leg volume). In contrast , insulin infusion after exercise significantly decreased the rate of prote in degradation (52 +/- 9 nmol . min(-1) . 100 ml(-1) leg volume). The insul in stimulatory effects on inward alanine transport and glucose uptake were three times greater during the postexercise recovery than at rest (P < 0.05 ). In contrast, the insulin effects on phenylalanine, leucine, and lysine t ransport were similar at rest and after exercise. In conclusion, the abilit y of insulin to stimulate glucose uptake and alanine transport and to suppr ess protein degradation in skeletal muscle is increased after resistance ex ercise. Decreased amino acid availability may limit the stimulatory effect of insulin on muscle protein synthesis after exercise.