Proteins of rat serum IV. Time-course of acute-phase protein expression and its modulation by indomethacine

changes in the concentration of major serum proteins were monitored from da y 0 to day 4 in three experimental groups: rats injected with turpentine, r ats receiving the turpentine shot and daily doses of indomethacine, and rat s given indomethacine alone. In inflamed animals, peak changes for acute-ph ase reactants, evaluated by two-dimensional electrophoresis (2-DE), were us ually observed between 48 and 72 h after the phlogistic stimulus. By itself , indomethacine was found to affect the synthesis of most proteins (except one of the thiostatin variants and ceruloplasmin); the changes in serum lev els, whether positive or negative, were the same as upon inflammation (exce pt for kallikrein-binding protein), but their extent and/or timing usually differed. When inflamed animals were given indomethacine, a clear-cut diffe rence in the concentration of some proteins was observed versus inflamed ra ts not given medication, at 24 h after the start of the treatments. Protein s mainly affected were alpha(2)-macroglobulin, alpha(2)-HS-glycoprotein, C- reactive protein and kallikrein-binding protein.*