Sn. Naryzhny et al., Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?, ELECTROPHOR, 20(4-5), 1999, pp. 1033-1038
It is assumed that DNA in mammalian cells is a dynamic conformationally uns
table system. This instability provides the cell with a mechanism for disso
ciating a large number of substances that bind tightly but not covalently t
o DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA
in the minor groove. We have selected cell lines with a high capability fo
r active dissociation of Hoechst 33342. Comparative protein analysis of the
se lines by means of two-dimensional (2-D) electrophoresis was performed. C
ell and nuclear proteins were analyzed from these and normal strains. A few
proteins with significantly changed quantities have been found. The prelim
inary search of the 2-D database allowed us to identity some known and unkn
own cellular proteins that could participate in active dissociation of the
dye from DNA.