A thermostable alpha-amylase producing maltohexaose from a new isolated Bacillus sp US100: study of activity and molecular cloning of the corresponding gene
M. Ben Ali et al., A thermostable alpha-amylase producing maltohexaose from a new isolated Bacillus sp US100: study of activity and molecular cloning of the corresponding gene, ENZYME MICR, 24(8-9), 1999, pp. 584-589
A Bacillus sp. US100 strain was isolated from Trunisian hot spring soil. Th
is strain secretes a thermoactive amylase having an optimal temperature of
82 degrees C and a remarkable thermostability with a half time of 40 min at
110 degrees C in the presence of 20% (w/v) of substrate. The main end prod
ucts of its action an starch are maltohexaose and maltopentaose which is th
e smallest substrate hydrolyzed by this enzyme. The molecular cloning, in E
. coli, of a 3-kb DNA fragment encoding far this activity is also reported
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