The mechanism of epristeride against benign prostatic hyperplasia

Epristeride, a 5 alpha-reductase inhibitor, decreases prostate size and imp roves symptoms in men with benign prostatic hyperplasia. However, little is known about the histopathology of the prostate after treatment with eprist eride. To study the relationship between apoptosis and the mechanism of epr isteride in the treatment of benign prostatic hyperplasia, the induction of apoptosis by epristeride was detected and measured in vitro by: (a) observ ing morphological changes in cells by light microscopy; (b) comparing the r elative content of dihydrotestosterone in the rat prostate epithelial cells untreated and treated with epristeride by microspectrophotometry; (c) esti mating changes in cell size and DNA integrity by flow cytometry; and (d) mo nitoring nucleosomal DNA fragmentation by agarose gel electrophoresis. The cells treated with epristeride showed a reduction in cell size, an increase in the cytoplasm/nuclear ratio, which is indicative of the condensation of nuclear chromatin, a significant decrease in optical density at 580 nm (OD 580 nm), and an oligonucleosomal ladder and a subdiploid peak of DNA charac teristic of apoptosis. Therefore, the mechanism of epristeride in the treat ment of benign prostatic hyperplasia might be apoptosis stimulated by decre asing dihydrotestosterone level. (C) 1999 Elsevier Science B.V. All rights reserved.