REGULATION OF THE RAG-1 PROMOTER BY THE NF-Y TRANSCRIPTION FACTOR

We have cloned the RAG-1 promoter region and have determined that almo st all detectable promoter activity resides within a 208-bp fragment, Sequence analysis of this promoter region has identified potential rec ognition motifs for a number of lymphocyte-restricted and ubiquitous t ranscription factors, Subsequent assays have revealed that the NF-Y tr anscription factor interacts with a CCAAT site within the RAG-1 promot er and appears to play an important role in the positive transcription al regulation of this gene.