Solid-state NMR triple-resonance backbone assignments in a protein

Authors
Tan, WM Gu, ZT Zeri, AC Opella, SJ
Citation
Wm. Tan et al., Solid-state NMR triple-resonance backbone assignments in a protein, J BIOM NMR, 13(4), 1999, pp. 337-342
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOMOLECULAR NMR
ISSN journal
09252738 → ACNP
Volume
13
Issue
4
Year of publication
1999
Pages
337 - 342
Database
ISI
SICI code
0925-2738(199904)13:4<337:SNTBAI>2.0.ZU;2-X
Abstract
Triple-resonance solid-state NMR spectroscopy is demonstrated to sequential ly assign the C-13' and N-15 amide backbone resonances of adjacent residues in an oriented protein sample. The observed C-13' chemical shift frequency provides an orientational constraint complementary to those measured from the H-1 and N-15 amide resonances in double-resonance experiments.