Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus

The genome of the nucleopolyhedrovirus (NPV) (T3 strain) pathogenic for Bom byx mori (Bm) was sequenced and analysed. The BmNPV genome was 128413 nucle otides long with a G+C content of 40% and contained 136 open reading frames (ORFs) encoding predicted proteins of over 60 amino acids. Although phenot ypically different, the genome organizations of BmNPV and Autographa califo rnica multinucleocapsid NPV (AcMNPV) were closely related. The BmNPV genome was over 90% identical to about three-quarters of the genome of AcMNPV. Th e relatedness of predicted amino acid sequences of corresponding ORFs betwe en BmNPV and AcMNPV was about 90%, However, the BmNPV genome lacked homolog ues of the following AcMNPV ORFs: Ac3 (conotoxin), Ac7 (orf603), Ac48 (etm) , Ac49 (pcna), Ac70 (hcf-1), Ac86 (pnk/pnl) and Ac134 (p94). In addition, B mNPV contained five ORFs related to Ac2. A high frequency of multiple 3 bp insertions was also found within BmNPV and AcMNPV coding sequences.