Cutting edge: Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor chemokine TECK

Thymus-expressed chemokine (TECK) has been reported to chemoattract dendrit ic cells, thymocytes, and activated macrophages. Here, we show that TECK is a specific agonist for a human orphan receptor called GPR-9-6, We have det ermined the cDNA sequence of human GPR-9-6 and cloned the corresponding mur ine cDNA, Human and murine GPR-9-6 expression is very high in the thymus an d low in lymph nodes and spleen. RT-PCR analysis of murine GPR-9-6 expressi on on murine FAGS-sorted thymocyte subpopulations showed that this gene is expressed in both immature and mature T cells, Additions of human or murine TECK to HEK 293/human GPR-9-6 and HEK 293/murine GPR-9-6 transfectants pro voked intracytoplasmic calcium mobilization. Human TECK also induced the in vitro migration of HEK 293/human GPR-9-6 cells. These results confirm that GPR-9-6 is a specific receptor for TECK, According to the established nome nclature system, we propose to rename GPR-9-6 as CC chemokine receptor 9 (C CR9).