Primary and secondary structure of the small-subunit ribosomal RNA of the naked, marine amoeba Vannella anglica: Phylogenetic implications

Citation
Gp. Sims et al., Primary and secondary structure of the small-subunit ribosomal RNA of the naked, marine amoeba Vannella anglica: Phylogenetic implications, J MOL EVOL, 48(6), 1999, pp. 740-749
Citations number
41
Categorie Soggetti
Biology,"Experimental Biology
Journal title
JOURNAL OF MOLECULAR EVOLUTION
ISSN journal
00222844 → ACNP
Volume
48
Issue
6
Year of publication
1999
Pages
740 - 749
Database
ISI
SICI code
0022-2844(199906)48:6<740:PASSOT>2.0.ZU;2-T
Abstract
The primary and secondary structure of the small-subunit ribosomal RNA (ssr RNA) gene from the naked, marine amoeba, Vannella anglica (subclass Gymnamo ebia), was determined. The ssrRNA is 1962 nucleotides in length, with a low G+C content of 37.1%. The ssrRNA is composed of several uncommon secondary structure features including helix E8-1, which may be a useful target for rRNA probes for the direct identification of isolates in mixed culture. Phy logenetic analysis of sequence data showed that V. anglica branched prior t o the rapid diversification of the eukaryotes, It did not associate with th e other naked, lobose amoebae represented by Acanthamoeba and Hartmannella, indicating that Vannella represents a separate amoeboid lineage and the su bclass Gymnamoebia is polyphyletic.