An approach for high sensitivity detection of prostate cancer by analysis of changes in structuredness of the cytoplasmic matrix of lymphocytes specifically induced. by PSA-ACT

Purpose: An alternative procedure for detection of prostate cancer was exam ined based on the observation that cells reexposed in vitro to antigenic or mitogenic stimulation will change their intracellular structuredness as me asured by polarization of fluorescent light emitted by labeled cells (SCM: test), Materials and Methods:Lymphocytes derived from patients bearing a nonmalign ant prostate tumor and healthy individuals were exposed to PSA-ACT,PHA, and MUC-1. Results: Of sixty-five patients with prostate carcinoma (CaP), sixty-two we re correctly diagnosed by the test. Of the eighty males in the control grou p, five were incorrectly diagnosed as having the disease and seventy-five w ere correctly diagnosed as healthy subjects. The sensitivity of the test wa s 96.8%. The specificity was 91.1%. The BPH (Benign Prostatic Hyperplasia) control group exhibited a sensitivity of 9.38%; but the specificity was 91. 1%. Similar percentages for specificity and sensitivity were observed in th e NRT (Non-Relevant Tumor) control group. Conclusions:The results shown here indicate the possibility of a different use of PSA-ACT for detection of prostate cancer with high specificity and s ensitivity.