D. Duijsings et al., Alfalfa mosaic virus RNAs serve as cap donors for tomato spotted wilt virus transcription during coinfection of Nicotiana benthamiana, J VIROLOGY, 73(6), 1999, pp. 5172-5175
Tomato spotted wilt virus (TSWV) was shown to use alfalfa mosaic virus (AMV
) RNAs as cap donors in vivo during a mixed infection in Nicotiana benthami
ana. By use of nested reverse transcription-PCR, TSWV N and NSs mRNAs provi
ded with capped leader sequences derived from all four AMV RNAs could be cl
oned and sequenced. The sequence specificity of the putative TSWV endonucle
ase involved is discussed.