Immunohistochemical analysis, human papillomavirus DNA detection, hormonalmanipulation, and exogenous gene expression of normal and dysplastic humancervical epithelium in severe combined immunodeficiency mice

The cervical squamocolumnar junction of normal and dysplastic human xenogra fts was maintained in SCID-beige mice. Dysplastic tissue maintained a dyspl astic morphology, irregular pattern of keratin expression, elevated levels of cellular proliferation, and human papillomavirus type 16 and/or type 18 DNA, Hyper plastic changes of normal xenografts occurred via high dose estr ogen exposure, and through recombinant adenovirus infection, the introducti on and stable expression of an exogenous gene was accomplished.