Morphometry and quality control for a May-Grunwald Giemsa stained preparation. A 40 centers cooperative study

A multicentric study including 40 medical centers has been done on May-Grun wald Giemsa (MGG) stain, to obtain precise information concerning the size, shape and color of cellular elements. A number of factors are taken into c onsideration including the zone of microscopic observation, the quality of staining, condition of grabbing images and the size of the sample. Morphome tric and colorimetric measurements have been applied as objective tools for demonstrating the predominance of each Red/Green/Blue component, and more particularly their reciprocal ratio. The results obtained for each center m ay constitute a guideline for adjusting their procedure, in order to reach a consensus regarding similar staining by all centers.