Analysis of salt-stress specific genes in Zygosaccharomyces rouxii by differential display

Salt-stress specific genes in Zygosaccharomyces rouxii detected by a differ ential display method were analyzed, Total RNAs were extracted from Z. roux ii cells cultured in YM broth containing 0% or 20% NaCl. cDNAs synthesized from total RNAs were amplified by PCR, and the PCR products were separated by polyacrylamide gel electrophoresis. Twenty-five genes specifically expre ssed in Z, rouxii cells cultured in the absence or presence of 20% NaCl wer e finally cloned into a TA vector and sequenced. Analysis of these genes re vealed that 13 genes had no sequence similarity and 12 genes had similarity to yeast genes present in the current GenBank/EMBL/DDBJ/PDB database searc hed with the BLAST program. Among 12 genes similar to, yeast genes, 10 gene s had similarity to Saccharomyces cerevisiae genes, one gene to a Candida a lbicans gene, and one gene was the Z. rouxii plasma membrane H+-ATPase gene .